Tell us whether you accept cookies

We use necessary cookies to make our website work. We also use cookies to collect information about how you use so we can improve our services.

Beta This is a new way of showing guidance - your feedback will help us improve it.

Soil organisms

Brexit: Transition period

The UK has now left the EU. Your health and safety responsibilities have not changed in the transition period.

Find the latest information on our Brexit pages


The soil risk assessment uses a soil predicted environmental exposure (PEC) calculated by Environmental Fate. For guidance about how to calculate the soil PEC see the Environmental Fate soil page.

Current Guidance Document

Guidance document on Terrestrial Ecotoxicology in the context of Directive 91/414/EEC (SANCO/10329/2002 rev.2, final) (pdf)


The standard study guidelines are:

Acute: OECD 207: Earthworm acute

Reproductive: OECD 222: Earthworm reproduction

Other soil invertebrates

The standard species are the Collembola, Folsomia candida and the predatory mite, Hypoaspis aculeifer . The standard study guidelines are:

Predatory mite: OECD 226: Predatory mite soil reproduction test

Collembolan: OECD 232: Collembola reproduction test in soil

Soil microorganisms

The standard study guidelines are:

OECD 216: Soil nitrogen transformation

OECD 217: Soil carbon transformation


Updated 2016-10-05